Pox & Papova

Finish Papilloma (Clinical, Pathophysiology)

Papova

Polyoma

Clinical

Virus found in urinary tract of immunocompromised patients

Usu. no disease

Progressive multifocal leukoencephalopathy

RareFatalDegenerativeDemyelinating diseaseLytic infection of oligodendrocytes (CNS)Immunocompromised patients (AIDS and reticuloendothelial malignancies)

Ureteral stenosis

Kidney transplant patients

Hemorrhagic cystitis

Bone marrow transplant patients

Diagnosis

Fetal glial cells

Routine cell culture

Immunoassay

Ag detection

Epidemiology

Papilloma

Characteristics

Cubic symmetry

100+ genotypes

Cell target

Epithelial cell

Skin keratinocyte

Mucous membranes

Induces epithelial cell proliferation

Replication linked to cell differentiation

Clinical

Warts

Common

Genital

Benign lesions

Often resolve spontaneously

Squamous cell carcinoma

Diagnosis

Detect viral DNA

PCR

in situ hybrid.

Southern blot

Perinuclear cytoplasmic vacuolization

Koilocytosis

Nuclear enlargement

Immunoassays

To detect viral Ag

Tissue culture

Epidemiology

Infects many different vertebrate animal species, but highly species-specific

Treatment

Lesion removal

Interferon

Antisense RNA???

Prevention

HPV Vaccine

Gardasil

Types(Associated with 90% of genital warts)611(Associated with 70% of cervical cancers)1618

Girls Age 9-18

Not yet recommended for women older than 26

Condoms

Pox

Characteristics

Classification

Ortho

Variola

Causes smallpox

Major

Mortality: 15-40%

Minor

Mortality: 1%

Vaccinia

Smallpox vaccine source

Monkeypox

ZoonoticGenerally confined to W. and Cent. Africa

Cowpox

Zoonotic

Para

Zoonotic infections

Orf

Zoonotic infection from sheep and goats

Milker's nodule

Unclassified

Molluscum contagiosum

Structure

Brick-shaped

Largest

Can be viewed with light microscopy

Most complex

Genome

Replication

Where does replication take place?What are sites of viral replication called?

Cytoplasm

Guarnieri bodies

Linear DS DNA

Encodes immune escape mechanisms

Clinical

Smallpox

Inhaled

Replication in UR tract

Flu-like symptoms

Vesicles form 4 days after onset

Orf, Cowpox, Monkeypox

Nodular lesions

Where are they found?What is the result?

Face, Fingers

Spontaneous resolution

Mollus. Contag.

Lesions

Nodular

Painless

Umbilicated

Molluscum bodies in tissue

Skin

Epidemiology

Transmission

Respiratory to lymphatics

Smallpox

Direct contact

Molluscum contagiosum

Usu. due to accidental exposure

Most infections by accidental exposure

Prevention

Vaccine for smallpox

Vaccinia virus