Image acquition

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Image acquition

spinning disk confocal

slow

better signal/noise ratio

for optical scetioning

bigger

light sensitive

single

smaller specimen, non-live imaging, living cells

sensitivity

less sensitive

higher

diameter can be changed

2D/3D imaging

rapid

array based detector

Subtopic

laser scanning confocal

scanning speed

very fast

multiple

specimen

bigger, live imaging

sensitive

more sensitive

photobleaching

reduced

pinhole

multiple pinholes

3D imaging

slower

detector

hybrid

photomultiplier