Kategorier: Alle - scanning - sensitivity - imaging

af Kabita Baral 5 år siden

222

Image acquition

In the realm of microscopy, advanced techniques like laser scanning confocal microscopy and spinning disk confocal microscopy are pivotal for imaging biological specimens. These methods offer distinct advantages and limitations.

Image acquition

Image acquition

spinning disk confocal

slow

better signal/noise ratio

for optical scetioning
bigger

light sensitive

single
smaller specimen, non-live imaging, living cells
sensitivity
less sensitive
higher
diameter can be changed
2D/3D imaging
rapid
array based detector
Subtopic

laser scanning confocal

scanning speed
very fast
multiple
specimen
bigger, live imaging
sensitive
more sensitive
photobleaching
reduced
pinhole
multiple pinholes
3D imaging
slower
detector
hybrid
photomultiplier

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