Initial question: What is the content and function of Y chromosomes?

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PCR on the region of interest with male and female DNA

RACE PCR and sequencing to assess the complete transcript sequence

Characterize the phenotype caused by the reduction of gene expression

Activate RNAi through injection of dsRNA on the appropriate developmental stage of the insect

Assess the gene expression reduction levels through qPCR

Characterize the function of Y genes

Where can we insert marker genes on the Y for population control purposes? (Sterile Insect Technique) We need a region where the marker will be stably expressed and easily integrated

What kind of genes exist on the Y?

Determine the relative expression of target genes through qPCR

Determine the tissue and time of gene expression

Isolate RNA from different developmental stages and tissues

Identify regions of the Y chromosome in the reference assembly that include genes

Validate that they are really on the Y

Theoretical interest

How do sex chromosomes evolve across species?

Practical interest

Which are the genes related to the sexual competitiveness of our organism?

Initial question: What is the content and function of Y chromosomes?