Jan 2013

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Electrical Apprentice Program

Rony Efendyにより

Applied Linguistics II

Johanna Vanegasにより

Economics system

Igor Glenderにより

Civil War Battles

Sherry Garrettにより

January 2013

Results

Outcomes

Expectations

Stimulated cells

Higher involvement in myotube formation

Alignment, perpendicular to direction of strain

Control

Unlikely >1 human nuclei per myotube

Low degree of fusion (<5%)

Measures

Degree of differentiation

Presence of Myosin

Morphology

No of syncytial nuclei

Human nuclei

Murine nuclei

Size

Directionality

Methodology

Mechanical Stimulation

Uniaxial cyclic tensile strain

Flexcell setup

6 well plates or the new design?

Cell line development

C2C12

C2C12-LifeAct-RFP

C2C12-GFP

ASC-GFP

Cellular stains

Myosin Heavy Chain

Primary [1:500]: Rabbit-anti-myosin

Secondary (10+10 ul/cm2): Zenon Alexa Fluor 647 mouse-anti-rabbit IgG

Cytoplasm

Green Fluorescent Protein

Ex: 487; Em: 509

Human Nuclei

Nuclear envelope

Primary [1:2000]: Monoclonal rabbit-anti-human Lamin

Secondary [1:400]: Alexa Fluor 555 conjugated goat-anti-rabbit

All Nuclei

DNA

Hoechst 33342

Introduction

Future Aspects

Conclusion

Discussion