B3: Pox & Papova

Pox & Papova

Finish Papilloma (Clinical, Pathophysiology)

Pox

Vaccine for smallpox

Vaccinia virus

Transmission

Usu. due to accidental exposure

Most infections by accidental exposure

Direct contact

Respiratory to lymphatics

Mollus. Contag.

Lesions

Skin

Molluscum bodies in tissue

Umbilicated

Painless

Nodular

Orf, Cowpox, Monkeypox

Nodular lesions

Where are they found?

What is the result?

Spontaneous resolution

Face, Fingers

Smallpox

Flu-like symptoms

Vesicles form 4 days after onset

Inhaled

Replication in UR tract

Genome

Encodes immune escape mechanisms

Linear DS DNA

Replication

Where does replication take place?

What are sites of viral replication called?

Guarnieri bodies

Cytoplasm

Structure

Most complex

Largest

Can be viewed with light microscopy

Brick-shaped

Classification

Unclassified

Molluscum contagiosum

Para

Zoonotic infections

Milker's nodule

Orf

Zoonotic infection from sheep and goats

Ortho

Cowpox

Zoonotic

Monkeypox

Vaccinia

Smallpox vaccine source

Variola

Causes smallpox

Minor

Mortality: 1%

Major

Mortality: 15-40%

Papova

Papilloma

Prevention

Condoms

HPV Vaccine

Girls Age 9-18

Not yet recommended for women older than 26

Gardasil

Types

Treatment

Antisense RNA???

Interferon

Lesion removal

Infects many different vertebrate animal species, but highly species-specific

Tissue culture

Immunoassays

To detect viral Ag

Koilocytosis

Nuclear enlargement

Perinuclear cytoplasmic vacuolization

Detect viral DNA

Southern blot

in situ hybrid.

PCR

Squamous cell carcinoma

Benign lesions

Often resolve spontaneously

Warts

Genital

Common

Characteristics

Replication linked to cell differentiation

Induces epithelial cell proliferation

Cell target

Mucous membranes

Skin keratinocyte

Epithelial cell

100+ genotypes

Cubic symmetry

Polyoma

Epidemiology

Diagnosis

Immunoassay

Ag detection

Routine cell culture

Fetal glial cells

Clinical

Virus found in urinary tract of immunocompromised patients

BK

Hemorrhagic cystitis

Bone marrow transplant patients

Ureteral stenosis

Kidney transplant patients

JC

Progressive multifocal leukoencephalopathy

Usu. no disease