Materiel and methods

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Materiel and methods

CELLS IN VIVO

A total of 29 rabbits were used for this experiment

the remaining nine rabbits were randomly divided into three groups for RNA-seq test:

20 rabbits were randomly divided into four groups for radiographic and histological tests

A 2 cm incision was made approximately 1 cm below the lower edge of the mandible body to expose the bone.

New Zealand rabbits were anesthetized using intravenous injections

CELLS IN VITRO

were differentiated from human embryonic stem cells

GM

freeze-dried GelMA (5% w/v final) was dissolved with the photoinitiator at 37 °C. GM was made by adding Matrigel to GelMA at the different volume ratios of 1:3 [GM (3:1)] or 1:5 [GM (5:1)].

RNA sequences

can identifie specific RNA as rRNA or tRNA or mRNA

can be use to identifie only a part of the RNA or the entire RNA

fresh samples were taken from the defect area

Total RNA was extracted using TRIzol , and RNA-Seq was performed by BioMarker

removed from the surrounding soft tissue, and were snap frozen in liquid nitrogen

PG

MATRIGEL + PIC exposed to UV radiation during 7 sec at 25°

PGM

MATRIGEL MODIFIED + PIC exposed to UV radiation during 7 sec at 25 °

PIC

In brief, in the presence of 0.05 mol % (relative to the monomer) 2-oxoglutaric acid under UV light irradiation (365 nm wavelength, 7.5 mW·cm−2) for 8 h, the precursor aqueous solutions of 1 mol·L−1 Sodium pstyrenesulfonate (NaSS) and 1 M 3- (methacryloylamino) propyl-trimethylammonium chloride (MPTC) were polymerizied and synthesized as PNaSS and PMPTC. PNaSS and PMPTC, respectively. Solutions with equal volume were slowly dripped into deionized water and stirred for 30 min, forming PIC precipitates, which were dissolved in sodium chloride solution to form the PIC solution.2