Multiplication of Bacteriophages

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Multiplication of Bacteriophages

Lysogenic Cycle

Important Results

3. specialized transduction: phage DNA and Bacterium DNA both in capsid

2. phage conversion: host cell may exhibit new properties

Vibrio cholerae

Clostridium botulinum

Corynebacterium diptheriae

1. cells are immune to reinfection

5. popping out of phage DNA and lytic cycle is started

4b. replicates the prophage DNA...prophage remains latent w/in the progeny cells

3b. circle can recombine and become part of bacterial DNA

every time bacterial DNA replicates so does the phage DNA

phage is now called prophage

4a. new phages produced and lysis occurs

3a. circle can multiply and transcribe

2. linear phage DNA forms a circle

1. penetration into E. coli cell

phage remains inactive

does not cause lysis and death of host cell

Lytic Cycle

5. Release: virions released from host cell

4. Maturation: bacteriophage DNA and capsids are assembled into complete virions

3. Biosynthesis: once bacteriophage DNA reaches cytoplasm of host cell, biosynthesis of viral nucleic acid and proteins occur

eclipse period: when infection virions are not present

2. Penetration: Bacteriophage DNA injected into bacterium

capsid remains outside

tail releases enzyme which breaks down portion of cell wall--phage lysozyme

1. Attachment: attachment site on virus fuses with receptor on bacterial cell

ends with lysis and death of host cell