Used lubricating engine oil used OTAL QUARTZ 5000 with SAE grade 20W-50
Then the soil samples are dried and homogenized.
Agricultural land is collected from automotive workshop in Ogbomoso, Oyo state
Kinetic Modelling and Half-Life Study on Bioremediation of Soil Co-Contaminated with Lubricating Motor Oil and Lead Using Different Bioremediation Strategies
The information is analyzed by ANOVA, at a level of 9 <0.05.
Periodic sampling of microorganisms is performed every 7 days for 42 days.
The microorganisms are incubated for 42 days.
Biodegradation of engine oil is expected.
Then it goes through a centrifuge
48 are expected to grow and then 1 ml of the culture is introduced at a concentration of lubricating engine (10% p/v)
The mixed bacterial culture (100 ml) is mixed with lubricating oil (3% p/v).
The flasks are prepared with Aeromonas, Micrococcus and Serratia, to mix in a sterilized test tube. A mixed bacterial culture is obtained.
The microorganisms for bioaugmentation were isolated.
