Microscopy Mind Map

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Acid-fast Staining

Gram Staining

Divides bacteria into two based on difference in cell wall structures

Gram Negative

Gram positive

MICROSCOPY

UNITS OF MEASUREMENT

Metric System

Micoorgansims and their structural components are measured in micrometers, nanometers and angstroms

Preparations of Specimens for Light Microscopy

Preparing Smears

Fixation

Staining

Smears

Wet Mount

Demonstrating motility of microorgansims

Electron Microscope

To study the surface features of cells and viruses

Employs a beam of electron in place pf light wave to produce magnified image

Fluorescence Microscope

Applications in medical microbiology

Specimens usually stained with flourochromes

Expose specimen to ultraviolet, violet or blue light

Phase Contrast Microscope

Stain is not necessary

Excellent way to observe living things

Converts differences in refractive index

Dark Field Microscope

Used to identify bacteria

Used to observe internal structures in eukaryotic micoorganisms

Produce a bright image of the object

Creates a sharp 3D image bybusing laser beam nd numerous applications including study of biofilms

Confocal Micoscopy

UV Microscope

Has shorter wavelength

Permits greater resolution

Microscope Parts

Body Tube

Objectives

Nosepiece

Substage

Stage Clips

Stage

Fine Adjustment Knob

Coarse Adjustment Knob

Light Source

Arm

Base

Special Staining

Negative Staining

Endospore Staining

Flagella Staining

Types of Staining

Differential Staining

Microscope is an instrument to observe microorgnisms

Light Microscope and Electron Microscope

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