作者：Aneesh Risbud 6 年以前
337
CELL DISRUPTION
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CELL DISRUPTION
CHEMICAL METHODS
ENZYME TREATMENT
USED FOR [RETREATMENT
EXPENSIVE AND PRESENCE COMPLICATE DOWNSTREAM
HYDROLYZE SPECIFIC BONDS BY ENZYMES
TRICODERMA
PENICILLIUM
MICROMONOSPORA
LEUCOCYTES
STREPTOMYCES
OSMOTIC SHOCK
EFFECT IS MINIMAL
CAUSES SUDDEN CHANGE IN SALT CONC.
DETERGENTS AND ALKALI TREATMENTS
ALKALI
ENZYME
20-30 MINS
pH- 11.5-12.5
HYDROLYSIS OF CELL WALL
DETERGENTS
PROTEIN DENATURATION
COMPOUNDS
TRITON X-100
SODIUM LAURYL SULPHATE
SDS
QUART AMMONIUM
DAMAGE LIPOPROTEIN
PHYSICO-MECHANICAL METHODS
ULTRASONICATION
VOLUMES UPTO 10 CM3 ARE EFFECTIVE
PROBES
SHORT RANGE
SHORT WORK LIFE
20 kHz VIBRATION LEADING TO CAVITATION AND SHOCK WAVES
FREEZE THAWING
SLOW , LIMITED RELEASE OF CELLULAR MATERIALS
THAWING CAUSES DISRUPTION
OF MICROBIAL CELL PASTE- ICE CRYSTAL FORMATION
AGITATION WITH ABRASIVES
DISSIPATION OVERCOME BY COOLING JACKET
BEADS- GLASS, ALUMINA CERAMICS AND TITANIUM
ROTATING DISCS AND SMALL BEADS IN A DISINTEGRATOR
SOLID SHEAR
DISRUPTION DUE TO ICE CRYSTALS
IDEAL FOR TEMPRATURE LABILE
PRESSURE EXTRUSION THROUGH ORIFICE AT -25 C
LIQUID SHEAR
WORKS ON PRINCIPLE OF PRESSURE DROP ACROSS VALVES
IMPINGES AGAINST OPERATIVE PRESSURE
HIGH PRESSURE HOMOGENIZERS EFFECTIVE FOR MICROBIAL DISRUPTION
LARGE SCALE ENZYME PURIFICATIOM
SLURRY PASSES THROUGH NO RETURN VALVES
